Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel

Isolation of exosome from culture medium in an effective way is desired for a less time consuming, cost saving technology in running the diagnostic test on cancer. In this study, we aim to develop an inertial microfluidic channel to separate the nano-size exosome from C666-1 cell culture medium as a selective sample. Simulation was carried out to obtain the optimum flow rate for determining the dimension of the channels for the exosome separation from the medium.
The optimal dimension was then brought forward for the actual microfluidic channel fabrication, which consisted of the stages of mask printing, SU8 mould fabrication and ended with PDMS microchannel curing process. The prototype was then used to verify the optimum flow rate with polystyrene particles for its capabilities in the actual task on particle separation as a control outcome. Next, the microchip was employed to separate the selected samples, exosome from the culture medium and compared the outcome from the conventional exosome extraction kit to study the level of effectiveness of the prototype.
The exosome outcome from both the prototype and extraction kits were characterized through zetasizer, western blot and Transmission electron microscopy (TEM). The microfluidic chip designed in this study obtained a successful separation of exosomes from the culture medium. Besides, the extra benefit from these microfluidic channels in particle separation brought an evenly distributed exosome upon collection https://joplink.net/exosome-isolation-kits/ while the exosomes separated through the extraction kit was found clustered together. Therefore, this work has shown the microfluidic channel is suitable for continuous separation of exosomes from the culture medium for a clinical study in the future.

Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer

Background: Breast cancer is the most common cancer, and the leading cause of cancer-related deaths, among females world-wide. Recent research suggests that extracellular vesicles (EVs) play a major role in the development of breast cancer metastasis. Axillary lymph node dissection (ALND) is a procedure in patients with known lymph node metastases, and after surgery large amounts of serous fluid are produced from the axilla. The overall aim was to isolate and characterize EVs from axillary serous fluid, and more specifically to determine if potential breast cancer biomarkers could be identified.
Methods: Lymphatic drain fluid was collected from 7 patients with breast cancer the day after ALND. EVs were isolated using size exclusion chromatography, quantified and detected by nanoparticle tracking analysis, electron microscopy, nano flow cytometry and western blot. The expression of 37 EV surface proteins was evaluated by flow cytometry using the MACSPlex Exosome kit.
Results: Lymphatic drainage exudate retrieved after surgery from all 7 patients contained EVs. The isolated EVs were positive for the typical EV markers CD9, CD63, CD81 and Flotillin-1 while albumin was absent, indicating low contamination from blood proteins. In total, 24 different EV surface proteins were detected.
Eleven of those proteins were detected in all patients, including the common EV markers CD9, CD63 and CD81, cancer-related markers CD24, CD29, CD44 and CD146, platelet markers CD41b, CD42a and CD62p as well as HLA-DR/DP/DQ. Furthermore, CD29 and CD146 were enriched in Her2+ patients compared to patients with Her2- tumors.
Conclusions: Lymphatic drainage exudate retrieved from breast cancer patients after surgery contains EVs that can be isolated using SEC isolation. The EVs have several cancer-related markers including CD24, CD29, CD44 and CD146, proteins of potential interest as biomarkers as well as to increase the understanding of the mechanisms of cancer biology.

Understanding the Role and Clinical Applications of Exosomes in Gynecologic Malignancies: A Review of the Current Literature

Background: Gynecologic malignancies are those which arise in the female reproductive organs of the ovaries, cervix, and uterus. They carry a great deal of morbidity and mortality for patients, largely due to challenges in diagnosis and treatment of these cancers. Although advances in technology and understanding of these diseases have greatly improved diagnosis, treatment, and ultimately survival for patients with gynecologic malignancies over the last few decades, there is still room for improvements in diagnosis and treatment, for which exosomes may be the key. This paper reviews the current knowledge regarding gynecologic tumor derived-exosomal genetic material and proteins, their role in cancer progression, and their potential for advancing the clinical care of patients with gynecologic cancers through novel diagnostics and therapeutics.
Literature review: Ovarian tumor derived exosome specific proteins are reviewed in detail, discussing their role in ovarian cancer metastasis. The key microRNAs in cervical cancer and their implications in future clinical use are discussed. Additionally, uterine cancer-associated fibroblast (CAF)-derived exosomes which may promote endometrial cancer cell migration and invasion through a specific miR-148b are reviewed. The various laboratory techniques and commercial kits for the isolation of exosomes to allow for their clinical utilization are described as well.
Conclusion: Exosomes may be the key to solving many unanswered questions, and closing the gaps so as to improve the outcomes of patients with gynecologic cancers around the world. The potential utilization of the current knowledge of exosomes, as they relate to gynecologic cancers, to advance the field and bridge the gaps in diagnostics and therapeutics highlight the promising future of exosomes in gynecologic malignancies.

Pathogenic Mechanisms of Preeclampsia with Severe Features Implied by the Plasma Exosomal miRNA Profile

Preeclampsia is a complication of pregnancy characterised by high blood pressure and organ damage after 20 gestational weeks. It is associated with high maternal and fetal morbidity and mortality; however, at present, there is no effective prevention or treatment for this condition. Previous studies have revealed that plasma exosomal miRNAs from pregnant women with preeclampsia could serve as biomarkers of pathogenic factors. However, the roles of plasma exosomal miRNAs in preeclampsia with severe features (sPE), which is associated with poorer pregnancy outcomes, remain unknown.
Thus, the aims of this study were to characterise plasma exosomal miRNAs in sPE and explore the related pathogenic mechanisms using bioinformatic analysis. Plasma exosomes were isolated using a mirVana RNA isolation kit.
The exosomal miRNAs were detected using high-throughput sequencing and the miRNAs related to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and gene ontology (GO) terms were analysed using the clusterProfiler package of R. Fifteen miRNAs exhibited increased expression and fourteen miRNAs exhibited reduced expression in plasma exosomes from women with sPE as compared to normal pregnant women.
Further, gene set enrichment analysis revealed that the differentially expressed plasma exosomal miRNAs were related to the stress response and cell junction regulation, among others. In summary, this study is the first to identify the differentially expressed plasma exosomal miRNAs in sPE. These findings highlight promising pathogenesis mechanisms underlying preeclampsia.

Exosome Isolation kit (for cell culture media)

- Ask for price

Exosome Isolation kit (for cell culture media)

- Ask for price

Exosome Isolation kit (for cell culture media)

- Ask for price

Exosome Isolation kit (for stem cell culture media)

- Ask for price

Exosome Isolation kit (for stem cell culture media)

- Ask for price

Reagent for Total Exosome Isolation (Culture Media Supplement)

50 ml 2485.2 EUR

VEX Exosome Isolation Reagent (from cell culture media)

50 ml 706.8 EUR

Exosome Isolation kit (for urine)

- Ask for price

Exosome Isolation kit (for urine)

- Ask for price

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

10 preps 664.8 EUR

GenMark ePlex NATtrol

In March, GenMark received EUA for its ePlex SARS-CoV-2 Test. Respiratory Pathogen Panel. Multiplex molecular diagnostic solutions provider GenMark Diagnostics has secured CE mark approval for its ePlex respiratory pathogen panel 2 (RP2). The ePlex RP has received FDA clearance for nasopharyngeal swab (NPS) specimens collected in viral transport media. COVID-19. ;

The RP2 Panel provides results in less than two hours for more than 20 viruses and bacteria that cause common respiratory infections with similar symptoms, including COVID-19, flu, bronchitis, and the common cold. Clinical implications of rapid eplex® respiratory pathogen panel testing compared to laboratory-developed real-time PCR Anneloes L. van Rijn, Roel H.T. Due to the batch-wise testing, laboratory-developed real-time polymerase chain reaction (PCR) assays (LDT) often result in a time to result of one day.

10/01/2019: Under Article Text added the third bullet point verbiage “For dates of service on or after 10/1/2019, laboratories billing for services using GenMark” ePlex Respiratory Pathogen (RP) Panel should report 0115U. The ePlex RP2 Control M451 is composed of synthetic DNA and RNA specifically designed for and intended to be used solely with the ePlex RP2 Panel on the ePlex System. In March, GenMark received EUA for its ePlex SARS-CoV-2 Test. Read More.

The ePlex respiratory pathogen panel (RP panel) is a novel molecular biology-based assay, developed by GenMark Diagnostics, Inc. (Carlsbad, CA), to be performed within a single cartridge for the diagnosis of 25 respiratory pathogens (viral and bacterial). in Top News. GenMarkâs ePLex-BCID Gram-Positive panel detects 20 bacterial targets, and the Fungal Pathogen panel detects 13 yeast pathogens in one and a half hours. AB Molecular Ltd. Butyl Road, Botolph Claydon, Respiratory Pathogen Panel. AB Molecular is the exclusive distributor in the UK for GenMark Dx and has been established to promote their innovative ePlex technology.

The ePlex RP2 Panel is designed for use with the companyâs ePlex system, which has been cleared by the FDA for use with the ePlex Respiratory Pathogen (RP) Panel and Blood Culture Identification (BCID) Panels (Gram-positive, Gram-negative and Fungal pathogens). The QIAstat-Dx® RP assay detected 312 of the 338 respiratory targets (92%) that were detected by the ePlex® RPP assay. This assay does NOT detect SARS CoV-2 (novel coronavirus) or MERS. Multiplex molecular panels provide high sensitivity (few false negatives) and specificity (few false positives) for multiple pathogens in ⦠GenMarkâs ePlex Respiratory Pathogen Panel 2 (RP2) achieves CE mark.

The company said RP2 drove the majority of the placements and revenues in Q3. Respiratory Panel 2 (RP2)] 0115U Respiratory infectious agent detection by nucleic acid (DNA and RNA), 18 viral types and subtypes and 2 bacterial targets, amplified probe technique, including multiplex reverse transcription for RNA targets, each analyte reported as detected or not detected [USE FOR GenMark ePlex Respiratory Pathogen (RP) Panel] SARS-CoV-2 (2 assays) Seasonal coronavirus.

We are very pleased to announce the 510(k) clearance of ePlex and the Respiratory Pathogen Panel. The new RP2 panel includes SARS-CoV-2, the pathogen that causes COVID-19. If the tube system is used, ensure specimens are in leak-proof containers that are securely closed and double bagged. ORIGINAL ARTICLE Clinical implications of rapid ePlex® Respiratory Pathogen Panel testing compared to laboratory-developed real-time PCR Anneloes L. van Rijn1 & Roel H. T. Nijhuis1 & Vincent Bekker 2 & Geert H.

Quality standards in respiratory real-life effectiveness research: the REal Life EVidence AssessmeNt Tool (RELEVANT): report from the Respiratory Effectiveness Group-European Academy of Allergy and Clinical Immunology Task Force.

Quality standards in respiratory real-life effectiveness research: the REal Life EVidence AssessmeNt Tool (RELEVANT): report from the Respiratory Effectiveness Group-European Academy of Allergy and Clinical Immunology Task Force.

A Task Force was commissioned collectively by the European Academy of Allergy and Clinical Immunology (EAACI) and the Respiratory Effectiveness Group (REG) to develop a high quality evaluation device for real-life observational analysis to establish high-quality real-life bronchial asthma research that might be thought-about inside future guideline growth.The ensuing REal Life EVidence AssessmeNt Tool (RELEVANT) was achieved by way of an intensive evaluation of present initiatives in this space.

The first model was piloted amongst 9 raters throughout 6 articles; the revised, interim, model underwent in depth testing by 22 reviewers from the EAACI membership and REG collaborator group, resulting in additional revisions and device finalisation. RELEVANT was validated by way of an evaluation of real-life effectiveness research recognized by way of systematic evaluate of Medline and Embase databases and regarding subjects for which real-life research might provide priceless proof complementary to that from randomised managed trials.

The subjects have been chosen by way of a vote amongst Task Force members and associated to the affect of adherence, smoking, inhaler machine and particle measurement on bronchial asthma remedy effectiveness.Although highlighting a basic lack of high-quality real-life effectiveness observational analysis on these clinically vital subjects, the evaluation offered insights into how recognized observational research may inform bronchial asthma pointers builders and clinicians.

Overall, RELEVANT appeared dependable and straightforward to make use of by professional reviewers.Using such high quality appraisal instruments is obligatory to evaluate whether or not particular observational real-life effectiveness research can be utilized to tell guideline growth and/or decision-making in medical observe.

 Quality standards in respiratory real-life effectiveness research: the REal Life EVidence AssessmeNt Tool (RELEVANT): report from the Respiratory Effectiveness Group-European Academy of Allergy and Clinical Immunology Task Force.
Quality standards in respiratory real-life effectiveness analysis: the REal Life EVidence AssessmeNt Tool (RELEVANT): report from the Respiratory Effectiveness Group-European Academy of Allergy and Clinical Immunology Task Force.

New European Academy of Allergy and Clinical Immunology definition on pollen season mirrors symptom load for grass and birch pollen-induced allergic rhinitis.

BACKGROUND
The use of allergen immunotherapy (AIT) for allergic rhinitis and its medical efficacy in medical trials will depend on the efficient dedication of pollen allergen publicity time durations. We consider pollen information from Germany to look at the new definitions on pollen season and peak pollen interval begin and finish as proposed by the European Academy of Allergy and Clinical Immunology (EAACI) in a just lately printed Position Paper. The goal was to reveal the potential of these definitions to reflect symptom hundreds for grass and birch pollen-induced allergic rhinitis primarily based on real-life information.

FBXW7 siRNA

20-abx916669
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

FBXW7 antibody

70R-6541 50 ug
EUR 560.4
Description: Rabbit polyclonal FBXW7 antibody raised against the C terminal of FBXW7

FBXW7 Antibody

1-CSB-PA822163LA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against FBXW7. Recognizes FBXW7 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

FBXW7 Antibody

1-CSB-PA623964
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against FBXW7. Recognizes FBXW7 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200

FBXW7 Antibody

DF12400 200ul
EUR 420

FBXW7 Antibody

1-CSB-PA251991
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against FBXW7. Recognizes FBXW7 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:10000, IHC:1:100-1:300

anti-FBXW7

YF-PA19671 50 ug
EUR 435.6
Description: Mouse polyclonal to FBXW7

anti-FBXW7

YF-PA19672 100 ug
EUR 483.6
Description: Rabbit polyclonal to FBXW7

anti-FBXW7

YF-PA26323 50 ul
EUR 400.8
Description: Mouse polyclonal to FBXW7

FBXW7 Antibody

F53267-0.08ML 0.08 ml
EUR 165

FBXW7 Antibody

F53267-0.4ML 0.4 ml
EUR 379

FBXW7 Antibody

F53304-0.08ML 0.08 ml
EUR 165

FBXW7 Antibody

F53304-0.4ML 0.4 ml
EUR 379

FBXW7 Blocking Peptide

33R-5108 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of FBXW7 antibody, catalog no. 70R-6541

FBXW7 Rabbit pAb

A5872-100ul 100 ul
EUR 369.6

FBXW7 Rabbit pAb

A5872-200ul 200 ul
EUR 550.8

FBXW7 Rabbit pAb

A5872-20ul 20 ul
EUR 219.6

FBXW7 Rabbit pAb

A5872-50ul 50 ul
EUR 267.6

FBXW7 Blocking Peptide

DF12400-BP 1mg
EUR 234

FBXW7 cloning plasmid

CSB-CL822163HU-10ug 10ug
EUR 846
Description: A cloning plasmid for the FBXW7 gene.

Anti-FBXW7 antibody

PAab03057 100 ug
EUR 494.4

anti- FBXW7 antibody

FNab03057 100µg
EUR 702
Description: Antibody raised against FBXW7

Anti-FBXW7 antibody

STJ29923 100 µl
EUR 332.4
Description: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Fbxw7 (1C11)

YF-MA18764 100 ug
EUR 435.6
Description: Mouse monoclonal to Fbxw7

pDONR223-FBXW7 Plasmid

PVTB00367 2 ug
EUR 427.2

Anti-FBXW7 (3D1)

YF-MA11557 100 ug
EUR 435.6
Description: Mouse monoclonal to FBXW7

FBXW7 Antibody, HRP conjugated

1-CSB-PA822163LB01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against FBXW7. Recognizes FBXW7 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

FBXW7 Antibody, FITC conjugated

1-CSB-PA822163LC01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against FBXW7. Recognizes FBXW7 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

FBXW7 Antibody, Biotin conjugated

1-CSB-PA822163LD01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against FBXW7. Recognizes FBXW7 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Human FBXW7 shRNA Plasmid

20-abx960632
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse FBXW7 shRNA Plasmid

20-abx974100
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

FBXW7 ELISA KIT|Human

EF009598 96 Tests
EUR 826.8

FBXW7 Recombinant Protein (Human)

RP039052 100 ug Ask for price

FBXW7 Recombinant Protein (Mouse)

RP134159 100 ug Ask for price

FBXW7 Recombinant Protein (Mouse)

RP134162 100 ug Ask for price

FBXW7 Recombinant Protein (Mouse)

RP134165 100 ug Ask for price

pECMV- 3*Flag- FBXW7

PVT10356 2 ug
EUR 361.2

Polyclonal Fbxw7 antibody - middle region

APR00709G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Fbxw7 - middle region. This antibody is tested and proven to work in the following applications:

FBXW7 ORF Vector (Human) (pORF)

ORF013018 1.0 ug DNA
EUR 114

Fbxw7 ORF Vector (Mouse) (pORF)

ORF044721 1.0 ug DNA
EUR 607.2

Fbxw7 ORF Vector (Mouse) (pORF)

ORF044722 1.0 ug DNA
EUR 607.2

Fbxw7 ORF Vector (Mouse) (pORF)

ORF044723 1.0 ug DNA
EUR 607.2

Polyclonal FBXW7 / FBW7 Antibody (N-Terminus)

APR02668G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FBXW7 / FBW7 (N-Terminus). This antibody is tested and proven to work in the following applications:

FBXW7 sgRNA CRISPR Lentivector set (Human)

K0767601 3 x 1.0 ug
EUR 406.8

Fbxw7 sgRNA CRISPR Lentivector set (Mouse)

K4531701 3 x 1.0 ug
EUR 406.8

Monoclonal FBXW7 Antibody (monoclonal) (M02), Clone: 3D1

AMM03534G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human FBXW7 (monoclonal) (M02). The antibodies are raised in mouse and are from clone 3D1. This antibody is applicable in WB and IHC, E

FBXW7 sgRNA CRISPR Lentivector (Human) (Target 1)

K0767602 1.0 ug DNA
EUR 184.8

FBXW7 sgRNA CRISPR Lentivector (Human) (Target 2)

K0767603 1.0 ug DNA
EUR 184.8

FBXW7 sgRNA CRISPR Lentivector (Human) (Target 3)

K0767604 1.0 ug DNA
EUR 184.8

Fbxw7 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4531702 1.0 ug DNA
EUR 184.8

Fbxw7 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4531703 1.0 ug DNA
EUR 184.8

Fbxw7 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4531704 1.0 ug DNA
EUR 184.8

FBXW7 Protein Vector (Human) (pPB-C-His)

PV052069 500 ng
EUR 577.2

FBXW7 Protein Vector (Human) (pPB-N-His)

PV052070 500 ng
EUR 577.2

FBXW7 Protein Vector (Human) (pPM-C-HA)

PV052071 500 ng
EUR 577.2

FBXW7 Protein Vector (Human) (pPM-C-His)

PV052072 500 ng
EUR 577.2

FBXW7 Protein Vector (Mouse) (pPB-C-His)

PV178882 500 ng
EUR 1278

FBXW7 Protein Vector (Mouse) (pPB-N-His)

PV178883 500 ng
EUR 1278

FBXW7 Protein Vector (Mouse) (pPM-C-HA)

PV178884 500 ng
EUR 1278

FBXW7 Protein Vector (Mouse) (pPM-C-His)

PV178885 500 ng
EUR 1278

FBXW7 Protein Vector (Mouse) (pPB-C-His)

PV178886 500 ng
EUR 1278

FBXW7 Protein Vector (Mouse) (pPB-N-His)

PV178887 500 ng
EUR 1278

FBXW7 Protein Vector (Mouse) (pPM-C-HA)

PV178888 500 ng
EUR 1278

FBXW7 Protein Vector (Mouse) (pPM-C-His)

PV178889 500 ng
EUR 1278

FBXW7 Protein Vector (Mouse) (pPB-C-His)

PV178890 500 ng
EUR 723.6

FBXW7 Protein Vector (Mouse) (pPB-N-His)

PV178891 500 ng
EUR 723.6

FBXW7 Protein Vector (Mouse) (pPM-C-HA)

PV178892 500 ng
EUR 723.6

FBXW7 Protein Vector (Mouse) (pPM-C-His)

PV178893 500 ng
EUR 723.6

FBXW7 3'UTR Luciferase Stable Cell Line

TU007813 1.0 ml
EUR 1825.2

Fbxw7 3'UTR Luciferase Stable Cell Line

TU106466 1.0 ml Ask for price

FBXW7 3'UTR GFP Stable Cell Line

TU057813 1.0 ml
EUR 1825.2

Fbxw7 3'UTR GFP Stable Cell Line

TU156466 1.0 ml Ask for price

Human F-box/WD repeat-containing protein 7 (FBXW7)

1-CSB-CF822163HU
  • EUR 1158.00
  • EUR 798.00
  • EUR 858.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Human F-box/WD repeat-containing protein 7(FBXW7) expressed in in vitro E.coli expression system

F-Box And WD Repeat Domain Containing 7 (FBXW7) Antibody

abx145824-100ug 100 ug
EUR 469.2

F-Box And WD Repeat Domain Containing 7 (FBXW7) Antibody

20-abx006803
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

F-Box And WD Repeat Domain Containing 7 (FBXW7) Antibody

20-abx124024
  • EUR 493.20
  • EUR 710.40
  • 100 ul
  • 200 ul

F-Box And WD Repeat Domain Containing 7 (FBXW7) Antibody

20-abx317508
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

F-Box And WD Repeat Domain Containing 7 (FBXW7) Antibody

abx233057-100ug 100 ug
EUR 661.2

FBXW7 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0767605 3 x 1.0 ug
EUR 451.2

Fbxw7 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4531705 3 x 1.0 ug
EUR 451.2

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Antibody

20-abx128045
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Antibody

20-abx129356
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

F-Box And WD Repeat Domain Containing 7 (FBXW7) Antibody (HRP)

20-abx317509
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

F-Box And WD Repeat Domain Containing 7 (FBXW7) Antibody (FITC)

20-abx317510
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

F-Box And WD Repeat Domain Containing 7 (FBXW7) Antibody (Biotin)

20-abx317511
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Antibody

20-abx212397
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Antibody

20-abx212888
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Goat F box/WD repeat containing protein 7(FBXW7) ELISA kit

E06F0338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat F box/WD repeat containing protein 7(FBXW7) ELISA kit

E06F0338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat F box/WD repeat containing protein 7(FBXW7) ELISA kit

E06F0338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit F box/WD repeat containing protein 7(FBXW7) ELISA kit

E04F0338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit F box/WD repeat containing protein 7(FBXW7) ELISA kit

E04F0338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit F box/WD repeat containing protein 7(FBXW7) ELISA kit

E04F0338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse F box/WD repeat containing protein 7(FBXW7) ELISA kit

E03F0338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse F box/WD repeat containing protein 7(FBXW7) ELISA kit

E03F0338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse F box/WD repeat containing protein 7(FBXW7) ELISA kit

E03F0338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig F box/WD repeat containing protein 7(FBXW7) ELISA kit

E07F0338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig F box/WD repeat containing protein 7(FBXW7) ELISA kit

E07F0338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig F box/WD repeat containing protein 7(FBXW7) ELISA kit

E07F0338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog F box/WD repeat containing protein 7(FBXW7) ELISA kit

E08F0338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog F box/WD repeat containing protein 7(FBXW7) ELISA kit

E08F0338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog F box/WD repeat containing protein 7(FBXW7) ELISA kit

E08F0338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey F box/WD repeat containing protein 7(FBXW7) ELISA kit

E09F0338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey F box/WD repeat containing protein 7(FBXW7) ELISA kit

E09F0338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey F box/WD repeat containing protein 7(FBXW7) ELISA kit

E09F0338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat F box/WD repeat containing protein 7(FBXW7) ELISA kit

E02F0338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat F box/WD repeat containing protein 7(FBXW7) ELISA kit

E02F0338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat F box/WD repeat containing protein 7(FBXW7) ELISA kit

E02F0338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human F- box/WD repeat- containing protein 7, FBXW7 ELISA KIT

ELI-27065h 96 Tests
EUR 988.8

Mouse F- box/WD repeat- containing protein 7, Fbxw7 ELISA KIT

ELI-20808m 96 Tests
EUR 1038

Human F box/WD repeat containing protein 7(FBXW7) ELISA kit

E01F0338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human F box/WD repeat containing protein 7(FBXW7) ELISA kit

E01F0338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human F box/WD repeat containing protein 7(FBXW7) ELISA kit

E01F0338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

FBXW7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K0767606 1.0 ug DNA
EUR 200.4

FBXW7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K0767607 1.0 ug DNA
EUR 200.4

FBXW7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K0767608 1.0 ug DNA
EUR 200.4

Fbxw7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4531706 1.0 ug DNA
EUR 200.4

Fbxw7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4531707 1.0 ug DNA
EUR 200.4

Fbxw7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4531708 1.0 ug DNA
EUR 200.4

Recombinant F-Box And WD Repeat Domain Containing Protein 7 (FBXW7)

4-RPJ890Hu01
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human F-Box And WD Repeat Domain Containing Protein 7 expressed in: E.coli

Recombinant F-Box And WD Repeat Domain Containing Protein 7 (FBXW7)

4-RPJ890Mu01
  • EUR 636.10
  • EUR 294.00
  • EUR 2055.36
  • EUR 765.12
  • EUR 1410.24
  • EUR 501.60
  • EUR 4958.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse F-Box And WD Repeat Domain Containing Protein 7 expressed in: E.coli

Human F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Protein

20-abx166205
  • EUR 844.80
  • EUR 343.20
  • EUR 2598.00
  • EUR 994.80
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Protein

20-abx166209
  • EUR 878.40
  • EUR 343.20
  • EUR 2766.00
  • EUR 1062.00
  • EUR 627.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human F-Box and WD Repeat Domain Containing 7 (FBXW7) ELISA Kit

abx387334-96tests 96 tests
EUR 1093.2

Guinea pig F box/WD repeat containing protein 7(FBXW7) ELISA kit

E05F0338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig F box/WD repeat containing protein 7(FBXW7) ELISA kit

E05F0338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig F box/WD repeat containing protein 7(FBXW7) ELISA kit

E05F0338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig F box/WD repeat containing protein 7(FBXW7) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

F-box and WD-40 domain protein 7 isoform 1 (FBXW7) polyclonal antibody

ABP-PAB-10563 100 ug Ask for price

F-box and WD-40 domain protein 7 isoform 2 (FBXW7) polyclonal antibody

ABP-PAB-10564 100 ug Ask for price

F-box and WD-40 domain protein 7 isoform 2 (FBXW7) polyclonal antibody

ABP-PAB-10565 100 ug Ask for price

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Human)

4-PAJ890Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human F-Box And WD Repeat Domain Containing Protein 7 (FBXW7)

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Mouse)

4-PAJ890Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse F-Box And WD Repeat Domain Containing Protein 7 (FBXW7)

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Human), APC

4-PAJ890Hu01-APC
  • EUR 414.00
  • EUR 3930.00
  • EUR 1094.40
  • EUR 528.00
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with APC.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Human), Biotinylated

4-PAJ890Hu01-Biotin
  • EUR 373.20
  • EUR 2952.00
  • EUR 872.40
  • EUR 457.20
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with Biotin.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Human), Cy3

4-PAJ890Hu01-Cy3
  • EUR 502.80
  • EUR 5190.00
  • EUR 1410.00
  • EUR 654.00
  • EUR 301.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with Cy3.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Human), FITC

4-PAJ890Hu01-FITC
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with FITC.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Human), HRP

4-PAJ890Hu01-HRP
  • EUR 379.20
  • EUR 3426.00
  • EUR 968.40
  • EUR 477.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with HRP.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Human), PE

4-PAJ890Hu01-PE
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with PE.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Mouse), APC

4-PAJ890Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with APC.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Mouse), Biotinylated

4-PAJ890Mu01-Biotin
  • EUR 379.20
  • EUR 3031.20
  • EUR 892.80
  • EUR 464.40
  • EUR 265.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with Biotin.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Mouse), Cy3

4-PAJ890Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with Cy3.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Mouse), FITC

4-PAJ890Mu01-FITC
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with FITC.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Mouse), HRP

4-PAJ890Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with HRP.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Mouse), PE

4-PAJ890Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with PE.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Human), APC-Cy7

4-PAJ890Hu01-APC-Cy7
  • EUR 685.20
  • EUR 7716.00
  • EUR 2046.00
  • EUR 912.00
  • EUR 382.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with APC-Cy7.

F-Box And WD Repeat Domain Containing Protein 7 (FBXW7) Polyclonal Antibody (Mouse), APC-Cy7

4-PAJ890Mu01-APC-Cy7
  • EUR 699.60
  • EUR 7932.00
  • EUR 2100.00
  • EUR 933.60
  • EUR 388.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse F-Box And WD Repeat Domain Containing Protein 7 (FBXW7). This antibody is labeled with APC-Cy7.
METHODS
Data coming from 4 pollen monitoring stations in the Berlin and Brandenburg space in Germany and for three years (2014-2016) have been used to analyze the correlation of season definitions, birch and grass pollen counts and complete nasal symptom and mediation scores as reported by sufferers in “Patients Hay fever Diaries” (PHDs). After the identification of pollen durations on the foundation of the EACCI standards, a statistical evaluation was employed, adopted by an in depth graphical investigation.
RESULTS
The evaluation revealed that the definitions of pollen season in addition to peak pollen interval begin and finish as proposed by the EAACI are correlated to symptom hundreds for grass and birch pollen-induced allergic rhinitis reported by sufferers in PHDs.
CONCLUSIONS
Based on our evaluation, the validity of the EAACI definitions on pollen season is confirmed. Their use is advisable in future medical trials on AIT in addition to in each day routine for optimum affected person care.

Antigens

Antigens are defined as substances capable of stimulating the production of antibodies, with which they react specifically. This definition is incomplete since it is well known that antigens can induce cell-mediated immune responses. So antigens are all molecules introduced into the body that can induce an immune response; that is to say the induction of the production of specific immune effectors (humoral or cellular) and to react with these.

antigen is a category of molecules (a molecular species) defined by its antigenic specificity. And, it defines antigenic specificity as the property of a given antigen to combine with a given (usually heterogeneous) population of antibodies. A given antigen can combine with several different or identical antibodies.

Classifications

According to origin

– Xeno-antigen XENO ANTIGENS: these are the antigens present in all individuals of one or more species distinct from that to which the immunized subject belongs.
– Allo antigens (Iso antigens): these are antigens found in a group of individuals of the same species and can induce an immune response in individuals who do not have them.
– Auto antigens: these are the antigens of an individual that can induce an anti-self immune response.

Depending on whether the production of antibodies depends on T cells or not

– Thymo-dependent antigens: are those against which the production of antibodies requires the help of T lymphocytes. This category is mainly represented by proteins.
– Thymo independent antigens: are those against which the production of antibodies does not require the help of T lymphocytes; such as polysaccharides and lipopolysacharides which are characterized by the presence of repetitive epitopes.

According to the chemical nature

 Protein antigens: these are the most immunogenic antigens. There are several types:
– Natural proteins: these are the main constituents of living things. They are encoded by corresponding genes.
– Artificial proteins: these are proteins whose natural core is on which side sequences are grafted.
– Synthetic proteins.

 Polysaccharide antigens: the immunogenic power of these antigens is weak. The simple polysaccharides have repetitive antigenic determinants which can activate B lymphocytes without resorting to T lymphocytes. The antigenic determinants of these antigens are sequential and nonconformational determinants, of approximately 6 sugars.

 Lipid antigens: lipids are not immunogenic. however, after their association with proteins, they can induce an immune response. They play the role of haptens. In certain diseases we find anti phospholipid antibodies.

 Nucleic acids: the immunogenicity of these substances is controversial when all attempts at immunization fail. However, immunization with nucleic acids associated with proteins induces the production of specific antibodies.

According to physical properties (solubility)

 Soluble antigens: constitute the majority of antigens in nature (proteins, polysaccharides, etc.)
 Particulate antigens: correspond to all particles, living or inert, which can induce an immune response (bacteria, virus, cell, parasite, etc.)

Applications

  1. vaccination
  2. serotherapy
  3. in vitro diagnosis
  4. skin tests
  5. desensitization

IMMUNOGLOBULINES

Our body has a defense system (immune system) ensuring its integrity.
It defends it against exogenous aggressions (microorganisms) and monitors pathological cell proliferation. To accomplish this task, it has natural (innate, non-specific) and other adaptive (acquired or specific) control mechanisms. The latter are provided by effectors which recognize antigens in a specific way.

What are immunoglobulines?

Immunoglobulins are serum glycoproteins from mammalian interstitial fluid that are produced by B cells (membrane immunoglobulin) or by plasma cells (soluble immunoglobulin). In general, it is produced following specific antigenic stimulation. Immunoglobulins produced after antigen stimulation or those having corresponding antigens are called ANTIBODIES.

CLASSES AND SUBCLASSES OF IMMUNOGLOBULINS

In mammals, there are five classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD) which differ in their Phi, the half-life, the number of constant domains and the role of the constant parts. The classes and subclasses are terminated by the types of heavy chains; each of these chains is encoded by a different gene.

1/ Immunoglobulin G (IgG)

it is a glycoprotein made up of two heavy chains (γ) and two light chains (κ or λ). It represents 70 to 75% of total immunoglobulins with a concentration of 10 to 18 g / l. She
includes four subclasses: IgG1, IgG2, IgG3 and IgG4 and which represent 66%, 23%, 7% and 4% of IgG respectively. The difference between them lies in the hinge region and in the number of disulfide bridges.
Half of these immunoglobulins are 21 days, except for IgG3 which is 7 days. Their sedimentation constant is 7 S and with a PM of 146 Kd. IgG (1,2,3) fix the complement. Only IgG1 and IgG3 which can cross the placenta. The γ chain consists of 3 constant domains and a variable. But all light chains are only formed from two domains, a constant and a variable.

2/ Immunoglobulin A (IgA)

it is a glycoprotein in two forms, a monomeric form consisting of two heavy chains (α) and two light chains (κ or λ) and represents 80% of serum IgA. The second form is predominant in secretions (milk, saliva …). It is dimeric of 385 Kd and 11 S. it contains the chain J of 15 Kd plus a secretory component of 70 Kd. IgA groups two subclasses:
IgA1 which is found, above all, in serum form and IgA2 which is found, above all, in secretory form. The concentration of serum IgA is 1 to 4.5 g / 1. They do not fix the complement and do not cross the placenta. The α chain consists of 3 constant domains and a variable.

3/ Immunoglobuline M (IgM)

it is a glycoprotein of 900 Kd and 19 S. Usually, it is pentameric and made up of 10 heavy chains (μ) and 10 light chains (κ or λ) identical. It represents 10% of serum immunoglobulins. The subunits are linked together by disulfide bridges and the chain J (junction). The μ chain contains four constant domains and one variable. The membrane IgM is monomeric containing an additional hydrophobic sequence which allows it to anchor to the cytoplasmic membrane. This molecule constitutes the specific receptor for the antigen on the B lymphocyte (BCR). It is associated with two molecules Igα (CD79a) and Igβ (C79b) which are responsible for signal transduction after antigenic stimulation.

4/ Immunoglobulin D

it is a glycoprotein consisting of two heavy chains (δ) and two light chains q (κ or λ) identical. It represents 10% of serum Ig. It exists in two forms; the serum form which is soluble and the membrane form which constitutes, with the membrane IgM, the BCRs of mature B lymphocytes. It is the most sensitive to proteolysis.
The heavy chains δ only bind to each other by a disulfide bridge. It is 184 kd, 7 S and a half-life of 3 days. The serum concentration is 0.05 to 0.4 g / l. the heavy chain has three constant domains and one variable.

5/ Immunoglobulins E

it is 190 Kd and 8S glycoprotein with a half-life of 3 days. It consists of two heavy chains (ε) and two light chains (κ or λ). Its serum concentration is 0.003 g / l. Most IgE are found to be fixed on basophils and mast cells by receptors specific to the constant part (RFCεI). It does not fix the complement and does not cross the placenta. It is a thermolabile Ig.

ANTIGENIC PROPERTIES

an immunoglobulin can induce an adaptive immune response (production of antibodies). So it has the properties of an antigen. The antibodies produced are directed against the various antigenic determinants located on the constant part or the variable part. There are three groups of antigenic determinants of light chains or heavy chains defining the isotype, allotype and idiotype.

1- Isotype: it is the properties of antigenic determinants which define the classes and subclasses of immunoglobulins of the same species. It is carried by the constant part.

2 – Allotype: it is the properties of antigenic determinants which differentiate different individuals within the same species. It is carried by the constant part.

3 – Idiotype: it is the properties of antigenic determinants which differentiate immunoglobulins of the same isotype and of same allotype in an individual. It is carried by the variable part.

ORGANIC ROLES

An immunoglobulin molecule consists of a variable part, represented by the Fab fragment which constitutes the site of attachment of the antigen to the antibody; the latter is called the PARATOPE. So the variable part is responsible for the recognition of the antigen and the reaction with it. The constant party represented by the FC plays several roles:
(1) Activation of the complement (IgG1, IgG2, lgG3).
(2) binding to receptors carried by cells derived from the hematopoietic line (cytophilia or cytotropy).
IgG have three receptors: RFCγI or CD 64, RFCγII or CD 32 and RFCγIII or CD 16. They mainly fix IgG l and IgG 3. They are expressed by monocytes, macrophages, polynuclear, NK and CTL. IgEs have two receptors: RFCε I which is expressed by mast cells and basophils and RFCεII or CD23 which is expressed by the majority of blood cells (monocyte; polynuclear, lymphocytes, platelets …).
(3) the catabolism of immunoglobulins whose speed is regulated by Fc and more precisely by CH2.
(4) certain isotypes of immunoglobulins have the property of crossing cells (trans cytosis). IgA is secreted in exocrine secretions and IgG is secreted in the interstitial fluid.

IMMUNE RESPONSE

The first contact of the antigen With the organism activates the immune cells (T and B lymphocytes) and induces a primary immune response which is characterized by:

  • The latency phase lasts several days
  • The Ig level is slightly increased and then declines rapidly
  • The isotype is IgM

The second contact induces a secondary immune response which is characterized by:

  • The latency phase is very short
  • the Ig level is very high
  • the isotype is IgG especially with also IgA and IgM

Applications

Diagnostic:
-Search for soluble or particulate antigens (invitro)
-Immuno-scintigraphy (invivo)
-Serodiagnosis
Therapeutic:
-Serotherapy
-IGIV.